A tentative exploration of multiplex methylation specific PCR for early diagnosis of nasopharyngeal carcinoma
10.3969/j.issn.1006-5725.2014.24.014
- VernacularTitle:多重MSP检测早期鼻咽癌甲基化基因的初步探索
- Author:
Yudong YE
;
Xianbin WANG
;
Qianhui QIU
- Publication Type:Journal Article
- Keywords:
Early nasopharyngeal carcinoma;
MSP;
Multiplex-MSP
- From:
The Journal of Practical Medicine
2014;(24):3919-3921
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of multiplex methylation specific PCR for early diagnosis of nasopharyngeal carcinoma. Methods The methylation status of 18 genes were verified by MSP from 35 early nasopharyngeal carcinoma andmultiplex-MSP was established through the optimized working condition for rapid detection of several genes′ methylation status. Results The methylation rate of 12 genes exceeds 50%. The methylation specific products in only one group (Segment U) did reach the expected results while those from the other three groups were consistent with the MSP′s. Conclusion Multiplex-MSP is an efficient and low-cost method, and it can produce ideal experimental results similar to those by the MSP and DNA microarrays.
