Effect of sphingosine-1-phosphate receptor 2 on coronary endothelial cells proliferation
10.3969/j.issn.1006-5725.2014.23.011
- VernacularTitle:2型1－磷酸鞘氨醇受体对冠脉内皮细胞增殖的影响
- Author:
Weihua LIU
;
Shaojun LIU
;
Huaina QIU
;
Jingxin GUO
;
Bin LIU
- Publication Type:Journal Article
- Keywords:
Sphingosine-1-phosphate;
Sphingosine-1-phosphate receptor 2;
Endothelial cells;
Proliferation
- From:
The Journal of Practical Medicine
2014;(23):3739-3741
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of sphingosine-1-phosphate receptor (S1PR2) in human coronary artery endothelial cell proliferation in vitro. Methods MTT assay was used to detect cell proliferation in human coronary artery endothelial after treatment of S1P and S1PR2 antagonist JTE-013. Phosphor-ERK and total- ERK level were measured by western blot in endothelial after treatment of S1P and JTE-013. Results 1 μmol/L S1P significantly increased endothelial cells proliferation. S1PR2 antagonist JTE-013 inhibited S1P-induced endothelial cell proliferation in dose-dependent manner. S1PR2 antagonist JTE-013 significantly inhibited S1P-induced phosphor-ERK level in endothelial cells. Conclusion S1PR2 may involve in S1P-induced endothelial cell proliferation through activation of ERK pathway.
