Function of JNK Signaling Pathway in Induction of Brain Ischemic Tolerance and Regulation of Shu-Xue Tong-Mai Capsule Pretreatment
10.11842/wst.2014.12.016
- VernacularTitle:JNK信号通路在脑缺血耐受诱导中的作用及疏血通脉胶囊预处理对其调控作用研究＊
- Author:
Tai LIU
;
Yuankan ZHANG
;
Deqing HUANG
;
Yueqiang HU
;
Ni LIANG
;
Yuying HU
;
Qianchao HE
;
Rongqun CHEN
;
Qingping ZHANG
- Publication Type:Journal Article
- Keywords:
Cerebral ischemic preconditioning;
cerebral ischemia reperfusion;
Shu-X ue Tong-Mai capsule;
JNK signaling pathway
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(12):2611-2615
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the function of c-Jun N-terminal kinase (JNK) signaling pathway in the induction of brain ischemic tolerance, and observe the function of Shu-Xue Tong-Mai (SXTM) capsule pretreatment. Ischemic preconditioning was performed for 3 min on rats to induce cerebral ischemic tolerance. Rat model of cere-bral ischemia reperfusion (the ischemia pretreatment group, I/R group) was established 24 h later. Western blot was used to detect the protein expression of JNK and phosphorylation of c-Jun N-terminal kinase (P-JNK), comparing to the expression with the sham operation group, I/R group and SXTM capsule group. Tunel method was applied to de-tect the apoptosis of neurons. Relationship between expression of JNK, P-JNK and apoptosis of neurons was also studied. The results showed that compared with the model group, expressions of P-JNK in ischemia preconditioning group and SXTM group were declined significantly (P < 0.05); and the apoptosis of neurons quantity was also de-clined (P< 0.05). It was concluded that ischemia preconditioning can decrease the apoptosis of neurons in cerebral ischemia reperfusion, and improve neurologic function. Its mechanism related to the inhibition of JNK signaling path-way. SXTM capsule pretreatment can protect the cerebral by inhibiting the JNK signaling pathway.
