Quantitative Determination of Agarotetrol in Agarwood by HPLC-DAD

VernacularTitle:HPLC-DAD测定沉香药材中沉香四醇的含量＊
Author: Yufan GU ; Qian ZHANG ; Huixia HUO ; Zheng HUANG ; Jing ZHANG ; Yunfang ZHAO ; Jun LI ; Pengfei TU
Publication Type:Journal Article
Keywords: Agarwood; agarotetrol; HPLC-DAD; quality control
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(12):2643-2646
CountryChina
Language:Chinese
Abstract: This study was aimed to establish a quantitative method for the determination of agarotetrol in agarwood to control its quality. The HPLC analysis was performed on a Diamonsil C18 column (4.6 mm í 250 mm, 5 μm), eluted with a mobile phase of water with 0.1% formic acid and acetonitrile in gradient mode with the flow rate of 0.7 mL·min-1. The detection wavelength was set at 252 nm. The column temperature was 30oC. The results showed that the separation of agarotetrol and adjacent peaks were more than 1.5, which achieved a baseline separation. A good lin-earity (r = 0.999 8) was observed in the range of 2.0-125.0 μg·mL-1, with the average recovery of 102.75%. The a-garotetrol cannot be less than 0.15% based on the data of Agarwood samples. It was concluded that the method was accurate and reliable to determine the content of agarotetrol, which can be used for the quality control of agarwood.