Effect of Celastrus Orbiculatus Extract on Epithelial-Mesenchymal Transition in HepG2 Cells
10.11842/wst.2014.12.022
- VernacularTitle:南蛇藤提取物对人肝癌细胞HepG2上皮间质转化的影响＊
- Author:
Yayun QIAN
;
Feng JIN
;
Ling CAO
;
Youyang SHI
;
Songhua LU
;
Xin JIANG
;
Lanfang JI
;
Yan YAN
;
Dan LI
;
Jun YONG
;
Yanqing LIU
- Publication Type:Journal Article
- Keywords:
Nan -She -Teng (Celastrus orbiculatus );
hepatic carcinoma;
epithelial -mesenchymal transition;
zebrafish;
signaling pathway
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(12):2647-2651
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of Nan-She-Teng (Celastrus orbiculatus) extract on epithe-lial-mesenchymal transition(EMT) in HepG2 cells. Except the control group, human hepatocellular carcinoma HepG2 cells in other groups were treated with Celastrus Orbiculatus extract in different concentrations (10, 20, 40, 80, and 160 μg·mL-1). The protein expression levels related to EMT were detected by western blotting. At 48 h after fertiliza-tion, the zebrafish embryos were randomly assigned to 7 groups as follows: untreated control group (E3 buffer), DMSO group (E3 buffer with 1% DMSO), and different dosages treatment of C.orbiculatus extract (10, 20, 40, 80, and 160μg·mL-1) for 24 h. The protein expressions of mTOR signaling pathways were detected by western blotting. The re-sults showed that compared with the control group, C.orbiculatus extract significantly increased E-cadherin protein expression. Simultaneously, C.orbiculatus extract inhibited vimentin and mTOR signaling pathways at protein levels. It was concluded that to a certain extent, C.orbiculatus extract prevented EMT in HepG2 cells by modulating the mTOR signaling pathway. Therefore, it suggested that mTOR can be chosen as a new therapeutic target for clinical treatment of hepatic carcinoma.
