Determination of Valepotriates and Their Degradation Products in Valeriana Jatamansi Jones by HPLC
10.11842/wst.2014.12.024
- VernacularTitle:HPLC法同时测定蜘蛛香药材中缬草三酯类化合物及其降解产物的含量＊
- Author:
Wenhui HOU
;
Yong LIU
;
Chunguo WANG
;
Guolin LIU
;
Qiantong LIU
;
Guoping CHANG
;
Jie LIU
;
Shuning SHI
;
Jinli SHI
- Publication Type:Journal Article
- Keywords:
Valeriana jatamansi Jones;
valtrate;
acevaltrate;
baldrinal;
11-ethoxyviburtinal;
HPLC
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(12):2658-2663
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish an HPLC method for simultaneous content determination of valtrate, acevaltrate, and their degradation products, which were baldrinal and 11-ethoxyviburtinal, in Valeriana jatamansi Jones. The separation and quantification of 4 constituents mentioned above were performed on an Agilent ZORBAX SB-C18 (4.6 mm×250 mm, 5 μm). The mobile phase consisted of water (A) - acetonitrile (B) with an optimized gradient program. The flow rate was 1 mL·min-1. The column temperature was set at 25℃. The wavelength was set at 241 nm. And the injection volume was 10μL. The results showed that among 14 different places of V. jatamansi, the 4 contents determined were different. The contents of valtrate, acevaltrate, and baldrinal in the Yunnan Baoshan Mount were the highest. And the content of 11-ethoxyviburtinal was the highest in Yunnan Dali. It was concluded that the method was with good precision, reproducibility and stability. And it was suitable for the determination of 4 valepotriates ingredients in V. jatamansi. It also provided references for the quality control and exploitation of V. jatamansi.
