Synthesis and Identification of Artificial Antigen for Alkaloids from Traditional Chinese Medicine
10.11842/wst.2014.12.025
- VernacularTitle:天然药物中生物碱类化合物人工抗原的合成及其鉴定方法＊
- Author:
Xiaolong ZHANG
;
Ting MA
;
Ting ZHANG
- Publication Type:Journal Article
- Keywords:
Alkaloids;
hapten;
artificial antigen;
coupling
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(12):2664-2670
- CountryChina
- Language:Chinese
-
Abstract:
Alkaloids are a kind of natural nitrogen compounds which are widespread in the nature, with special and significant activities in pharmacology. In recent years, immunoassay techniques have been applied for the qualitative and quantitative analysis of alkaloids from traditional Chinese medicine (TCM). However, preparation and identifica-tion of artificial antigen with good immunogenicity are necessary and important for establishment of immunoassay for alkaloids from TCM. This paper summarized the designing ideas and procedures for preparation of artificial antigen and identification for the conjugates of alkaloids and carrier protein, in order to provide reference point for establish-ment and application of immunoassay techniques.
