TLC Identification of Pomegranate Seed and Terminalia Chebula, HPLC Determination of Cinnamal and Piperine in Tibetan Medicine Shi-Wei Xiao-Shi-San
10.11842/wst.2014.12.034
- VernacularTitle:藏药十味消食散中石榴子、诃子的TLC鉴别及桂皮醛、胡椒碱的HPLC测定＊
- Author:
Peng CHEN
;
Yuanhui LIU
;
Guifa LUO
;
Xingfu ZHANG
- Publication Type:Journal Article
- Keywords:
Shi-Wei Xiao-Shi-San;
pomegranate seed;
Terminalia chebula;
cinnamal;
piperine;
TLC;
HPLC
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(12):2720-2724
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish a method for quality control of Tibetan medicine Shi-Wei Xiao-Shi-San (SWXSS). Thin layer chromatography (TLC) was established for the qualitative determination. Cinnamal and piperine were determined by HPLC. The separation was performed on Phenomenex C18 column (4.6 mm × 250 mm, 5μm). The results showed that TLC can be used in the identification of pomegranate seed and Terminalia chebula. The linear ranges of cinnamal and piperine were within the range of 0.06-0.37μg and 0.05-0.33μg, respectively. And the standard line was Y=9.273 2×106X-2.348 2×105, r=0.999 8;Y=7.315×103X-3.857, r=1.000 0. It was concluded that the identification method was specific, accurate and practical, which can be applied in the quality control of SWXSS.
