Determination of Octylacetate in Jing-Fu-Kang Granules by GC

VernacularTitle:GC法测定颈复康颗粒中乙酸辛酯的含量＊
Author: Can ZHANG ; Shuqing DUAN ; Yunxia LI
Publication Type:Journal Article
Keywords: Jing-Fu-Kang granules; GC; essential oil; olibanum; octylacetate; content determination
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(12):2731-2734
CountryChina
Language:Chinese
Abstract: This study was aimed to establish a method for the determination of octylacetate in Jing-Fu-Kang (JFK) granules. The content was determined by GC with Agilent HP-5 (30 m × 0.32 mm × 0.25μm) capillary column, with hydrogen flame ionization (FID) as detector. The injector temperature was 220℃. The detector temperature was 260℃. The split ratio was 20:1. At first, the temperature should be maintained to 80℃for 2 min, and then heating up to 200℃with the speed of 10℃·min-1. This temperature was maintained for 10 min. The results showed that the good linear relationship of octylacetate was within the range of 0.156 5-7.824 2 mg·mL-1, r=0.999 9. The average recovery rate was 100.00%, and RSD was 1.91%(n=9). The lowest detection limit of octylacetate was 1.633 6 ng. It was concluded that the established method was simple and reproducible with no interference. It can be applied to the quality control of JFK granules.