Significance and dynamic expression of P-glycoprotein in status epilepticus rats
10.3969/j.issn.1006-5725.2014.23.010
- VernacularTitle:P－糖蛋白在大鼠癫痫持续状态表达的动态变化及其意义
- Author:
Han YU
;
Zhen DENG
;
Miaoshan LUO
;
Yuanshu ZHAO
;
Liu XIE
;
Li CHEN
;
Xiaoqin ZHU
;
Jingxin HU
;
Shuisheng LEI
- Publication Type:Journal Article
- Keywords:
Status epilepticus;
P-glycoprotein;
Pentylenetetrazol;
Drug Resistance protein
- From:
The Journal of Practical Medicine
2014;(23):3735-3738
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the dynamic expression of the drug resistance protein P-glycoprotein (P-gp) within 72 hours in the pentylenetetrazol (PTZ)-induced status epilepticus (SE) model, and to identify the optimal detection time to inhibit P-gp. Methods mRNA and protein expressions of P-gp in rats hippocampal tissue were detected by using immunohistochemistry , RT-qPCR and Western blot at different time points after modeling (0, 3, 6, 12, 24, 48, 72 h). Results The mean density of P-gp protein in the hippocampus of status epilepticus model was 0.325 1 ± 0.008 2 at 24 h, and was 0.396 3 ± 0.016 8 at 48 h, which were consistently higher than those of the control group (P < 0.05, P < 0.01, respectively). Results of qRT-PCR showed that MDR1a expression was significantly upregulated at 24 h and at 48 h (P < 0.05, P < 0.01, respectively). Western blot assay revealed that P-gp protein was also significantly increased at 48 h after seizures (P < 0.05). Conclusions The upregulation of P-gp after SE peaked at 48 h, which maybe the optimal detection time to detect drug resistant after SE.
