Methodological Research on TLC Identification of Radix Mirabilis himalaica

VernacularTitle:藏药喜马拉雅紫茉莉的薄层鉴别方法学研究＊
Author: Hui LIN ; Ting ZHAO ; Qingjia REN ; Huiqin ZOU ; Jiahui LI ; Lian PENG ; Zhiyu REN ; Yonghong YAN
Publication Type:Journal Article
Keywords: Tibetan medicine; Radix Mirabilis himalaica; TLC identification; quality control
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(12):2725-2730
CountryChina
Language:Chinese
Abstract: This study was aimed to establish the TLC identification method of Radix Mirab ilis himalaic a. The β-sitosterol and daucosterol were used as the reference substances. The single-factor test was used. A variety of factors which affected TLC were systematically investigated to filter out the best TLC conditions for identification of different batches of medicines. The results showed that the best TLC conditions were as follows: silica gel G plates, extraction solvent (methanol), reagent (5% sulfuric acid in ethanol), extraction method (ultrasonic extraction with methanol), ex-tracted time (30 min), the agent (petroleum ether-ethyl acetate-acetone (5:2:1)) and sample volume (6 μL). It was concluded that the method, which had high separation degree, was reproducible and simple. It can be used as the quality control of Radix Mirab ilis himalaic a.