Determination of Tetrodotoxin in Marine Organisms by High Performance Liquid Chromatography-Mass Spectrometry and Cleaned up by Immunoaffinity Column
10.11895/j.issn.0253-3820.140704
- VernacularTitle:免疫亲和柱净化-液相色谱-串联质谱法测定海洋生物中河豚毒素
- Author:
Zhongyong YAN
;
Xiaojun ZHANG
;
Qifu LI
;
Ying WANG
;
Jiapeng LIU
;
Ju LONG
;
Yin ZHU
;
Huicheng YANG
- Publication Type:Journal Article
- Keywords:
Immunoaffinity column;
High performance liquid chromatography-mass spectrometry;
Marine organisms;
Tetrodotoxin
- From:
Chinese Journal of Analytical Chemistry
2015;(2):277-281
- CountryChina
- Language:Chinese
-
Abstract:
A method was developed for the determination of tetrodotoxin in marine organisms by high perfor-mance liquid chromatography-mass spectrometry with immunoaffinity column. The samples were extracted with 1% acetic acid methanol solution and diluted with phosphate buffer at pH 7-8. After cleaned up by immuno-affinity column, the samples were analyzed by LC-MS/MS and quantitatively determined by external standard method. The chromatographic separation was performed on an ACQUITY UPLC BEH Amide column with gradient elution by using acetonitrile and 5 mol/L ammonium acetate solution containing 0. 1% formic acid as mobile phase. Detection was carried out by electrospray positive ionization mass spectrometry in the multiple reaction monitoring mode. Linear ranges of TTX was in the range of 0. 3 -20. 0 μg/L with correlation coeffi-cient more than 0. 997. The quantification limit of the method was 0. 3 μg/kg. The recoveries of standard addition for tetrodotoxin were 88. 7%-102. 3%, and the relative standard deviation was 2. 0%-6. 4%. The method could be used to identify and quantify tetrodotoxin in marine organisms with satisfactory reproducibility and sensitivity.
