Qualitative and Quantitative Analysis of Bushen Jiannao Grains by Ultra Performance Convergence Chromatography
10.11895/j.issn.0253-3820.140655
- VernacularTitle:超高效合相色谱法定性与定量分析补肾健脑颗粒
- Author:
Ruijuan ZHU
;
Bo WANG
;
Fang HU
;
Can LI
;
Xiaoya LIU
;
Shilan FENG
;
Wei ZHOU
- Publication Type:Journal Article
- Keywords:
Ultra performance convergence chromatography;
Bushen Jiannao Grains;
Echinacoside;
β-Ecdyserone
- From:
Chinese Journal of Analytical Chemistry
2015;(2):288-293
- CountryChina
- Language:Chinese
-
Abstract:
An ultra performance convergence chromatographic ( UPC2 ) method was established for the attribution analysis of the main peaks as well as the quantitative determination of echinacoside andβ-ecdyserone in Bushen Jiannao Grains. The samples were extracted with ethanol and separated on Waters ACQUITY UPC2TM BEH column (100 mm × 3. 00 mm, 1. 7 μm), with a gradient supercritical CO2-0. 05%phosphoric acid-methanol solvent system at 40 ℃. The flow rate was 0. 8 mL/min, the detection wavelength was set at 248 nm and the injection volume was 1 μL. Results showed that all the main peaks in the fingerprint were clearly attributed. The peak named 12 wasβ-ecdyserone with the content of 380μg/g and the peak named 15 was echinacoside with the content of 9. 562 mg/g. The method was simple, eco-friendly, accurate and reliable compared with HPLC and UPLC.
