Detection of chicken embryo lethal orphan virus and egg drop syndrome virus by multiplex polymerase chain reaction

VernacularTitle:鸡胚致死孤儿病毒和鸡减蛋综合症病毒多重PCR 检测方法的建立及初步应用
Author: Shujing WANG ; Rui FU ; Xiaobo LI ; Ji WANG ; Li WEI ; Wei GONG ; Bingfei YUE ; Zhengming HE
Publication Type:Journal Article
Keywords: Chicken embryo lethal orphan virus(CELO); Egg drop syndrome virus(EDS); Avian adenovirus
From: Chinese Journal of Comparative Medicine 2015;(1):66-70
CountryChina
Language:Chinese
Abstract: Objective To establish multiplex PCR assay for detection of chicken embryo lethal orphan virus (CELO)and egg drop syndrome virus (EDS).Methods According to GenBank gene sequence , two pairs of specific primers designed were amplified CELO long fiber protein and EDS hexon protein gene sequence .The specificity and sensitivity of multiplex PCR were tested .We also use the multiplex PCR to detect exogenous CELO and EDS in influenza virus.Results Two target bands have been successfully amplified and verified by sequencing .The specificity of the method is better , and the sensitivity is 10 -4μg/mL.The results of detecting exogenous CELO and EDS in 12 influenza virus were negative .Conclusion The multiplex PCR assay for detection of CELO and EDS was established successfully , which have good specificity and high sensitivity , and have high value and application prospect for detecting exogenous CELO and EDS in influenza virus .