Protective effects of glutamine pretreatment on occludin protein in rats with intestinal ischemia-reperfusion injury
10.3969/j.issn.1000-4718.2015.02.031
- VernacularTitle:谷氨酰胺对大鼠肠缺血再灌注损伤后闭合蛋白的保护作用
- Author:
Aili WANG
;
Qiong NIU
;
Ning SHI
;
Xingfang JIA
;
Chengxia LIU
- Publication Type:Journal Article
- Keywords:
Glutamine;
Intestinal ischemia-reperfusion injury;
Occludin;
Inflammation;
Oxidative stress
- From:
Chinese Journal of Pathophysiology
2015;(2):364-368
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To determine the effects of glutamine ( Gln) pretreatment on occludin protein in the rats with intestinal ischemia-reperfusion ( I/R ) injury.METHODS: Male Wistar rats ( n =30 ) were randomly divided into 3 groups (n=10):sham group, I/R group and Gln pretreatment group.The rats in Gln pretreatment group were pretreated with Gln at dose of 1 g? kg-1? d-1 by orogastric route for 7 d, and those in the other 2 groups were pretreated with the same volume of normal saline .Intestinal I/R was induced by 30-min occlusion of the superior mesenteric artery followed by 24 h of reperfusion.After the operation, the levels of IL-10, IL-2, TNF-α, SOD and MDA were measured.The occludin protein was determined by the methods of immunohistochemistry and Western blotting .RESULTS: The occludin protein level in I/R group was significantly lower than that in sham group and Gln group (P<0.05).The levels of MDA and TNF-αin I/R group were significantly higher than those in sham group and Gln group ( P<0.05 ) .The levels of SOD , IL-10 and IL-2 in I/R group were significantly lower than those in sham group and Gln group ( P<0.05 ) .CONCLUSION:Glutamine has a protective effect on occludin protein in intestinal ischemia-reperfusion injury .The mechanism may be rela-ted to oxidative stress response and inflammatory inhibition .
