Study on the differen sterilizaion processes of High performance liquid chromatography to the metronidazole injection
10.3969/J.ISSN.1672-8270.2015.02.011
- VernacularTitle:高效液相色谱仪对甲硝唑注射液灭菌工艺筛选研究
- Author:
Fengxia LI
;
Fengru WANG
;
Hongwei LI
- Publication Type:Journal Article
- Keywords:
High performance liquid chromatography;
Metronidazole;
Content determination
- From:
China Medical Equipment
2015;(2):37-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective: By high performance liquid chromatography determination of metronidazole content of different sterilization parameters metronidazole injection, selection of the optimal sterilization parameters. Methods: The Agilent Extend-C18 analytical column (250 mm×4.6 mm, 5μm) was adopted with the mobile phase of water:Alcohol (80:20) at a flow rate of 1.5 mL/min; The column temperature was at 30℃; The detection wavelength was at 320 nm, 121℃ 15 min, the content of effective components metronidazole injection in 115℃ 30 min sterilization parameters. Results:The average content of metronidazole determination before sterilization liquid is divided into 101.57%, with excessive killing sterilization after liquid content is 101.47%, determined by the probability of survival after sterilization liquid content of 101.60%. The content of active component of different sterilization parameters of metronidazole and before sterilization contrast difference. Conclusion: The method is fast rapid, convenient, in accordance with the level of risk decision trees screened Metronidazole injection sterilization using terminal over kill sterilization accurate and can be used for the metronidazole in metronidazole injection.
