Simultaneous Determination of Four Ingredients in Xielining Tablets by HPLC

VernacularTitle:HPLC 梯度洗脱法同时测定泻痢宁片中秦皮甲素秦皮乙素黄芩苷和鞣花酸含量
Author: Caihua WEN
Publication Type:Journal Article
Keywords: Xielining tablets; Aesculin; Aesculetin; Baicalin; Ellagic acid
From: Herald of Medicine 2015;(3):384-387
CountryChina
Language:Chinese
Abstract: Objective To develop a HPLC method for determination of aesculin, aesculetin,baicalin and ellagic acid in Xielining tablets. Methods The hypersil C18 column was used with the flow rate of 1. 1 mL·min-1 . The mobile phase A consisted of methanol-acetonitrile(4∶1),the mobile phase B consisted of 0. 1% phosphoric acid solution; The detection wave-lengths were λ1 =334 nm(aesculin and aesculetin),λ2 = 280 nm(baicalin),and λ3 = 254 nm(ellagic acid). Results There was a good linear relationship between the peak area values and concentrations of aesculin,aesculetin,baicalin and ellagic acid. The quantitation range of aesculin,aesculetin,baicalin and ellagic acid was 0. 058 6 -1. 172 0 μg( r = 0. 999 2), 0. 015 4 -0. 308 0 μg(r=0. 999 8),0. 447 2-8. 944 0 μg(r=0. 999 6),and 0. 072 6-1. 452 0 μg(r=0. 999 5), respectively. The aver-age recovery was 97. 24% (RSD=0. 78% ),97. 76% (RSD=1. 11% ),98. 43% (RSD=0. 93% ) and 96. 89% (RSD=0. 62% ), respectively. Conclusion The method is convenient,accurate,sensitive,reproducible and may be used in the determination of aesculin, aesculetin,baicalin and ellagic acid in Xielining tablets.