Study on EDTA disk potentiation test for detecting metallo-β-lactamase of Acinetobacter baumannii
10.3969/j.issn.1673-4130.2015.05.002
- VernacularTitle:EDTA 纸片增效试验检测鲍曼不动杆菌金属β-内酰胺酶
- Author:
Youhua HE
;
Qingwan YAO
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
metallo-β-lactamase;
EDTA disk potentiation test
- From:International Journal of Laboratory Medicine
2015;(5):579-580
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research a method for detecting metallo-β-lactamase(MBL)to provide the basis for rationally selecting antibacterial drugs in clinic.Methods A total of 104 strains of imipenem-resistant Acinetobacter baumanmii(IRAB)were collected. The MBL phenotype was detected by using the EDTA disk potentiation test and the detection results were compared with the PCR detection results of MBL.Results Among 104 strains of tested IRAB,21 strains were the MBL gene positive by using PCR;24 strains were the MBL gene positive by using the EDTA disk potentiation test.Compared with the PCR detection results,the sensi-tivity of the EDTA disk potentiation test was 95.2% and the specificity was 95.1%.Conclusion The EDTA disk potentiation test for detecting MBL of Acinetobacter baumanmii is simple,credible in the detection results and cheap in cost,which is suitable for the preliminary identification of the MBL-producing Acinetobacter baumanmii in the clinical microbiology laboratory of the primary hos-pitals.
