Effect of hydrogen sulfide on myocardial fibrosis and PPARγand NF-κB expression in rat model of diabetes
10.3969/j.issn.1000-4718.2015.04.011
- VernacularTitle:硫化氢对糖尿病大鼠心肌纤维化及 PPARγ、NF-κB 表达的影响
- Author:
Ting XIAO
;
Jian LUO
;
Zhixiong WU
;
Fang LI
;
Jingjing ZHANG
;
Jun YANG
- Publication Type:Journal Article
- Keywords:
Hydrogen sulfide;
Diabetic myocardial fibrosis;
Type I collagen;
Peroxisome proliferator-activa-ted receptorγ;
Nuclear factor-κB
- From:
Chinese Journal of Pathophysiology
2015;33(4):635-639
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To explore the effects of hydrogen sulfide ( H2 S) on the myocardial fibrosis in a rat model of diabetes and its mechanism.METHODS:Single intraperitoneal injection of streptozotocin ( STZ) was utilized to establish a rat model of diabetes.Sodium hydrosulfide was used as an exogenous donor of hydrogen sulfide.Male SD rats were ran-domly divided into control group, STZ group, STZ+H2 S group and H2 S group.Eight weeks later, HE and VG staining methods were used to observe the collagen distribution and collagen volume fraction was measured by image analysis.The expression levels of type I collagen, PPARγand NF-κB in the cardiac tissues were determined by Western blotting.RE-SULTS:Compared with control group, collagen distribution and the expression levels of type I collagen and NF-κB in the cardiac tissues were markedly increased (P<0.05), while PPARγwas significantly decreased in STZ group (P<0.05), but these indexes were reversed significantly in STZ+H2S group (P<0.05).The expression levels of type I collagen, PPARγand NF-κB had no significant difference between H2 S group and control group.CONCLUSION:Hydrogen sulfide attenuates cardiac fibrosis in diabetic rats, and its mechanism may be related to PPARγ-NF-κB signaling pathway.
