Paeoniflorin inhibits functional responses of rat cerebellar Purkinje cells to acute hypoxia insult
10.3969/j.issn.1000-4718.2015.04.016
- VernacularTitle:芍药苷抑制大鼠小脑浦肯野细胞对急性缺氧的功能反应
- Author:
Yingge REN
;
Xiaoli TAN
;
Jing CHEN
;
Jing ZHANG
;
Yongping DU
;
Yueping ZHANG
- Publication Type:Journal Article
- Keywords:
Paeoniflorin;
Hypoxia;
Purkinje cells;
Membrane potential;
Synaptic transmission
- From:
Chinese Journal of Pathophysiology
2015;33(4):664-668
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To investigate the effects of paeoniflorin ( Pae) on the functional responses induced by acute hypoxic insult in the rat cerebellar Purkinje cells ( PCs) .METHODS:The whole-cell patch clamp was used for the intra-cellular recording of PCs in the rat cerebellar slices to evaluate the changes of membrane potential, the excitability of PCs, and the parallel fibre ( PF)-PC excitatory postsynaptic currents ( EPSCs) upon acute hypoxic insult alone or with the pre-sence of Pae.RESULTS:PCs showed an initial hyperpolarization followed by brief depolarization and long lasting post-hy-poxia hyperpolarization after hypoxia exposure.Pae completely blocked hypoxia-induced hyperpolarization and decreased the amplitude and the duration of hypoxic depolarization.Hypoxia up-regulated the excitability of rat PCs.Pae didn’t show any significant effect on the hypoxia-induced hyperexcitability in PCs.Acute hypoxia induced long-term depression ( LTD) in rat cerebellar PF-PC EPSCs, and Pae partially reversed hypoxia-induced depression in PF-PC EPSCs.CONCLUSION:Pae significantly suppresses hypoxia-induced responses in rat PCs and probably increases the tolerance of rat PCs to acute hypoxia.
