Construction of a Single Chain Variable Fragment Antibody Against Sulfonamides and Its Interaction with Sulfathiazole
10.11895/j.issn.0253-3820.140629
- VernacularTitle:磺胺类药物单链抗体的制备及其与磺胺噻唑相互作用研究
- Author:
Yonghan LI
;
Kai WEN
;
Xuezhi YU
;
Chenglong LI
;
Zhanhui WANG
- Publication Type:Journal Article
- Keywords:
Protein-ligand interaction;
Homology modeling;
Molecular docking;
Single chain variable fragment antibody;
Sulfathiazole
- From:
Chinese Journal of Analytical Chemistry
2015;(3):366-370
- CountryChina
- Language:Chinese
-
Abstract:
The interaction between the antibody and the corresponding target molecule determines the characteristics of immunoassay. In this study, a single chain variable fragment antibody (scFv4C7) derived from the hybridoma strain 4C7 were prepared via genetic engineering technique. The recognition properties of scFv4 C7 was determined and compared to those of the parent monoclonal antibody by indirect competitive enzyme-linked immunosorbent assay(ic-ELISA). Three dimensional structure of the scFv4C7 was presented by Swiss-Model, and sulfathiazole ( STZ) was docked to the scFv4C7 model to obtain the structure of the binding complex. The results from the ic-ELISA showed that the binding properties of scFv4C7 were comparable with the parent monoclonal antibody and STZ was almost completely buried in a deep binding pocket formed by the heavy chain and light chain of the antibody. The interaction between STZ and scFv4C7 was more closely related to the heavy chain and the complementarity-determining region ( CDR ) H3 loop played more important role than other CDR loops. The study preliminary provided the necessary structural information for the preparation of antibody with broader specificity and higher affinity.
