Discussion on Improving Purity of Primary Myocardial Cells from Neonatal Rats
10.11842/wst.2015.02.007
- VernacularTitle:提高新生大鼠原代心肌细胞纯度方法的探讨
- Author:
Xinlu WANG
;
Lin CUI
;
Youping WANG
;
Bin LI
;
Shiyang XIE
;
Mingjun ZHU
- Publication Type:Journal Article
- Keywords:
Neonatal rat;
myocardial cell;
primary culture;
identification
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(2):333-337
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the method to improve the purity of primary myocardial cells from neonatal rats. The myocardial tissues of rats which were born 1-3 days were repeated digested by 0.08% myocardial cells digestive juices. And then, cells were neutralized with DMEM medium which containing 10% fetal bovine serum. The cells were separated with differential sidewall ion. The red blood cell cracking liquid was added to remove the red blood cells. Bromine deoxidization uracil (Brdu) was added to purify the myocardial cells. Then, cells were incubated in the CO2 incubator for two days. The serum-free synchronization was performed for one day. The results showed that the output of myocardial cells from one rat was about 1.2 × 106. The dynamic myocardial cells occupied more than 90%. The purity of myocardial cells was more than 90%. After 3 to 4 days, the cell fusion of myocardial cells was formed with spontaneous rhythm beats. It was concluded that the method can ensure the yield and the activity of the myocardial cells. At the same time, the purity of myocardial cells can also be improved greatly.
