Determination of Cinnamic Aldehyde Content in Xianggui Huazhuo Capsules by Gas Chromatography-Mass Spectrometry
10.3870/yydb.2015.03.025
- VernacularTitle:气相色谱-质谱法测定香桂化浊胶囊中桂皮醛的含量
- Author:
Dan WU
;
Chenchen ZHANG
;
Jizhang YANG
;
Hongmiao LIU
- Publication Type:Journal Article
- Keywords:
Xianggui Huazhuo capsules;
Cinnamic aldehyde;
Gas chromatography-mass spectrometry;
Content deter-mination
- From:
Herald of Medicine
2015;(3):376-378
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determining cinnamic aldehyde content in Xianggui Huazhuo capsules by gas chromatography-mass spectrometry (GC-MS). Methods The content of cinnamic aldehyde was determined by GC-MS. Separation was performed on a capillary column (30 m×0. 25 mm, 0. 25 μm) with HP-5 as the stationary phase. A programmed temperature was employed. The flow rate was 1 mL·min-1 with He as carrier gas, and split ratio was 50∶1. The injection volume was 1. 0 μL. Results The cinnamic aldehyde was well isolated from the other ingredients. A good linear relationships of cin-namic aldehyde in range of 0. 02-4. 00 mg·mL-1 was observed. The correlation coefficient was 0. 999 4. The average recovery of cinnamic aldehyde was 96. 2% , and RSD was less than 2. 11% . Conclusion The method is simple, accurate and suitable for determination of cinnamic aldehyde content.
