Bumetanide inhibits tumor-cell proliferation by down-regulating NKCC1 expression
10.7644/j.issn.1674-9960.2015.07.002
- VernacularTitle:布美他尼对肿瘤细胞增殖的抑制作用及其与NKCC1表达的相关性
- Author:
Chen XU
;
Handong WEI
;
Ying JIANG
- Publication Type:Journal Article
- Keywords:
bumetanide;
NKCC1;
cell proliferation;
antineoplastic agents
- From:
Military Medical Sciences
2015;(7):495-498
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the application of bumetanide to inhibition of tumor cell proliferation.Methods In different cell lines, the expression of natrium,kalium, chloride cotransporter 1 ( NKCC1) was detected by Western blotting while the proliferation of different tumor cells was examined by CCK-8 kit.Results The target protein NKCC1 expression in lung cancer cell line ( A549 ) and colorectal cancer cell line ( HCT116 ) was significantly higher than that in chronic myelogenous leukemia cell line (K562), esophageal cancer cell line (Eca109), cervical carcinoma cell line (HeLa), T lymphocytic leukemia cell line (Jurkat) and breast cancer cell line (MCF7).IC50 Values of bumetanide were significantly lower in A549 and HCT116 than in K562, Eca109,HeLa,Jurkat and MCF7.Furthermore, the inhibiory rate and the target protein expression level were positively correlated.Conclusion Bumetanide can inhibit tumor cell proliferation and NKCC1 can serve as a potential target of anticancer drugs.
