Effect of epithelial-to-mesenchymal transition on the invasion and migration abilities of lung squamous cell carcinoma
10.3969/j.issn.1000-8179.20142037
- VernacularTitle:上皮间充质转化对肺鳞状细胞癌侵袭和迁移能力的影响
- Author:
Yanjun ZHENG
;
Baocun SUN
;
Xiulan ZHAO
;
Xu ZHENG
;
Yanlei LI
;
Zhiqiang QIU
;
Qiang GU
;
Xueyi DONG
;
Yanhui ZHANG
- Publication Type:Journal Article
- Keywords:
epithelial-to-mesenchymal transition;
lung squamous cell carcinoma;
TGF-β1;
invasion;
migration
- From:
Chinese Journal of Clinical Oncology
2015;(5):265-270
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the clinical significance of epithelial-to-mesenchymal (EMT) in lung squamous cell carcino-ma (LSCC) and to examine the effect of EMT on the invasive and migration abilities of LSCC. Methods:Immunohistochemical stain-ing was performed to determine the expression of E-cadherin, Vimentin, and TGF-β1 in 79 LSCC patients, and the clinical significance was explored. SK-MES-1 lung squamous carcinoma cells were cultured in conditioned medium containing various concentrations of transforming growth factor-β1 (TGF-β1) for 5 and 10 days. The expression levels of E-cadherin and Vimentin were detected via West-ern blot and reverse transcription-polymerase chain reaction (RT-PCR). With different concentrations and induction times, invasion and wound healing assays were performed to evaluate the invasion and migration abilities. Results:E-cadherin expression was significantly lower, whereas Vimentin expression was significantly higher in LSCC with lymph node metastasis than in that without noda metastasis (P<0.05). In the tissues of 79 LSCC patients, TGF-β1 expression was significantly related to lymph node metastasis (P<0.05). Western blot showed that Vimentin expression was higher, whereas E-cadherin expression was lower in TGF-β1 inducing medium with 10 ng/mL SK-MES-1 cells than in the other media. RT-PCR showed similar results. Scratch test and invasion assay both showed that treat-ment of cells with cytokines markedly enhanced the migration and invasion of the cells. Conclusion:Lymph node metastasis of LSCC correlates with EMT. SK-MES-1 cells undergo EMT via TGF-β1 induction, which enhances invasion and migration.
