Inhibiting effect of alcohol extract from Dioscore bulbifera on gastric cancer cells
10.11958/j.issn.0253-9896.2015.02.006
- VernacularTitle:黄药子醇提物抑制胃癌细胞功能的研究
- Author:
Leilei WANG
;
Dandan WANG
;
Guanhong CHEN
;
Xue KONG
;
Liwen ZHENG
;
Jianing WANG
- Publication Type:Journal Article
- Keywords:
Dioscore bulbifera;
stomach neoplasms;
cell proliferation;
colony-forming units assay;
cell migration assay
- From:
Tianjin Medical Journal
2015;(2):133-136
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibiting effects of alcohol extract from Dioscore bulbifera on proliferation, colony formation and migration of cancer cell lines. Methods Alcohol extract from Dioscore bulbifera was prepared using Soxhlet extraction. Human gastric cancer cell line MGC803 was treated with different concentrations(0, 60, 120 mg/L)of al?cohol extract from Dioscore bulbifera. In vitro, proliferation, colony formation and migration of gastric cancer cells were detect?ed by MTT, colony formation experiments and Transwell assay respectively. Results The proliferation(day2-day 6, F=29.130, 21.864, 67.826, 36.015, 43.656, P<0.01)and colony formation(F=11.918,P<0.01)of gastric cancer cells were significantly inhibited by administration of alcohol extract from Dioscore bulbifera at both 60 mg/L and 120 mg/L . The migra?tion(F=4.258,P<0.05)of gastric cancer cells were significantly suppressed after cells were treated with120 mg/L alcohol ex?tract from Dioscore bulbifera. Conclusion Alcohol extract from Dioscore bulbifera significantly inhibit proliferation, colony formation and migration of gastric cancer cells.
