Curcumin induces M1 phenotype derived from murine RAW264.7 macrophages polarization to alternatively activated M2 phenotype
10.7652/jdyxb201502022
- VernacularTitle:姜黄素促进 RAW264.7源性 M1巨噬细胞向替代激活 M2表型极化
- Author:
Fangyuan CHEN
;
Zuyi YUAN
;
Juan ZHOU
;
Huan WANG
;
Li XUE
;
Ning GUO
- Publication Type:Journal Article
- Keywords:
curcumin;
RAW264.7 macrophage;
macrophage polarization;
M1/M2 phenotype;
PPARγ;
LPS;
IFNγ
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(2):257-262
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:Objective To observe the effect of curcumin on RAW264.7 macrophages induced with LPS and IFNγ(M1)and the mechanisms involved.Methods Curcumin of different concentrations (6.25 μmol/L,12.5μmol/L and 25 μmol/L)was used to treat RAW264.7 macrophages induced with LPS and IFNγ(M1)for 12 h,and RAW264.7 macrophages induced with LPS and IFNγ(M1)were incubated with 20μmol/L GW9662 and 25 μmol/L curcumin for 12 h.Using Real-time PCR,ELISA and Western blotting analysis,we examined the expressions of IL-1β,IL-6,PPARγand phenotype markers M2 (KLF4,FIZZ1,and MGL1 )and the expressions of KLF4 and FIZZ1 when PPARγwas inhibited.Results Curcumin of different concentrations all could inhibit the expressions of IL-1βand IL-6 in RAW264.7 macrophages induced with LPS and IFNγ(M1).Curcumin of different concentra-tions could upregulate the expression of M2 markers (KLF4,FIZZ1 and MGL1)and PPARγin RAW264.7 macro-phages induced with LPS and IFNγ(M1).When M1 macrophages were incubated with curcumin and GW9662,the expression of the M2 phenotype markers was reduced.Conclusion Curcumin polarized the M1 phenotype macro-phages derived from RAW264.7 macrophages to become M2 phenotype through activating PPARγ.
