Determination of Entrapment Efficiency of Loratadine Binary Ethosomes by Sephadex Microcolumn Com-bined with HPLC
- VernacularTitle:葡聚糖凝胶微柱-HPLC测定氯雷他定二元醇质体包封率
- Author:
Chai JUN
;
Chen YING
;
Li KAI
- Publication Type:Journal Article
- Keywords:
Loratadine;
Binary ethosomes;
Entrapment efficiency;
Gel microcolumn centrifugation
- From:
China Pharmacist
2015;(2):244-246
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method to determine the entrapment efficiency of loratadine binary ethosomes. Methods:The gel microcolumn centrifugation method was employed to separate the free drug from loratadine binary ethosomes. The content of lorata-dine was determined by HPLC to calculate the entrapment efficiency. Results: A calibration linear curve of loratadine concentration was within the range of 10. 2-102. 0 μg·ml-1(r=0. 999 5). The average entrapment efficiencies of three batches of loratadine binary ethosomes were 86. 75%, 87. 26% and 86. 00%, respectively. Conclusion:The method is simple and rapid, and can be used to de-termine the entrapment efficiency of loratadine binary ethosomes accurately.
