Comparison of biological characteristics of human gingival junctional epithelial cells and oral epithelial cells
10.3969/j.issn.1671-167X.2015.01.006
- VernacularTitle:人牙龈结合上皮细胞和口腔龈上皮细胞生物学特性的比较
- Author:
Jinsheng ZHONG
;
Fang MEI
;
Hongquan ZHANG
- Publication Type:Journal Article
- Keywords:
Gingiva;
Epithelial cells;
Cell adhesion;
Cell proliferation;
Cell movement
- From:
Journal of Peking University(Health Sciences)
2015;(1):32-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To isolate P-cadherin positive and negative oral gingival epithelial cells , and to compare the biological characteristics with junctional epithelial cells .Methods:Human oral gingival epi-thelial cells and junctional epithelial cells were cultured .P-cadherin positive and negative cells were iso-lated from oral gingival epithelial cells .The cellular adhesion , proliferation and migration were measured and compared .Results:The P-cadherin positive cells accounted for 20%of oral gingival epithelial cells . Compared with juctional epithelial cells , P-cadherin positive oral gingival epithelial cells showed similar properties of adhesion and migration , and stronger proliferation ability (0.72 ±0.06 vs.0.60 ±0.05, P<0.05).P-cadherin negative oral gingival epithelial cells showed weaker ability of adhesion (48%± 6%vs.87%±11%, P<0.05), proliferation (0.36 ±0.04 vs.0.60 ±0.05, P<0.05) and migration (10.3 ±2.7 vs.23.4 ±4.8, P<0.05).Conclusion: P-cadherin positive oral gingival epithelial cells showed some similar but different biological characteristics , compared with juctional epithelial cells , which suggested that during the process of transforming oral gingival epithelial cells into juctional epithelial cells, complex gene and protein changes were involved instead of simply cellular migration .
