Determination of Gallic Acid,Quercetin and Kaempferol in Gongyanping Capsules by HPLC
- VernacularTitle:HPLC法同时测定宫炎平胶囊中没食子酸、槲皮素、山柰素的含量
- Author:
Liu MIN
;
Yu LE
- Publication Type:Journal Article
- Keywords:
Gongyanping capsules;
Gallic acid;
Quercetin;
Kaempferol
- From:
China Pharmacist
2015;(2):310-312
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of gallic acid,quercetin and kaempferol in Gongyanping capsules. Methods:HPLC was applied with the chromatographic conditions as follows: The chromatographic column was Agilent-SB C18 (250 mm × 4. 6 mm,5 μm) at 30℃; the mobile phrase was methanol-0. 3% phosphoric acid solution with gradient elution; the flow rate was 1. 0 ml·min-1. Results: The linearity relationship of gallic acid, quercetin and kaempferol was within the range of 98.200-491.00 μg·ml-1(r=0.999 9), 7.520-37.600 μg·ml-1(r=0.999 9) and 4.940-24.700 μg·ml-1(r=0.999 9), re-spectively;the average recovery was 96. 74%(RSD=1. 33%), 98. 18%(RSD=1. 70%) and 97. 04%(RSD=1. 28%),respective-ly. Conclusion:The method is simple, accurate and repeatable.
