Different viral vectors in gene-enhanced bone tissue engineering
10.3969/j.issn.2095-4344.2015.02.021
- VernacularTitle:基因强化骨组织工程中的病毒载体
- Author:
Zhentao XIAO
;
Zhongkai GUO
;
Lixin GUO
- Publication Type:Journal Article
- Keywords:
Genes;
Viruses;
Gene Therapy
- From:
Chinese Journal of Tissue Engineering Research
2015;(2):272-276
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Various gene-delivery strategies have be used to transfer targeted genes into damaged bone tissues. As the most efficient gene vector, viral delivery systems have been used in bone tissue engineering research. OBJECTIVE: To thoroughly discuss the applications of different viral vectors in gene-enhanced bone tissue engineering. METHODS:A computer-based online search was performed in PubMed database for the related articles from January 2002 to January 2015. This review centered on viral vector transduction methods and their use in bone tissue engineering. Adenovirus, retrovirus, adeno-associated virus and chimeric virus were al discussed. Advantages and limitations of different vectors and their applicability toward bone tissue engineering were presented in this article. A total of 24 articles were included for review. RESULTS AND CONCLUSION:Current viral vectors for gene delivery in gene-enhanced bone tissue engineering are summarized, including recent work where combinatorial gene therapy is used to express groups of genes to stimulate bone regeneration. Future directions for this field are discussed, where viral vectors mediated gene expression systems wil be combined with cels such as mesenchymal stem cels seeded in synthetic scaffolds to repair bone loss. Gene-enhanced bone tissue engineering has more advantages than traditional tissue engineering; viral vectors contribute to higher gene transfection efficiency than normal vectors. Long-term clinical observation is needed for the safety of viral vectors used in gene-enhanced tissue engineering in the body. Viruses are stil the most efficient means by which exogenous genes can be introduced into seeds cels.
