Spatiotemporal expression pattern of E-cadherin and P-cadherin during mouse tooth development
10.3969/j.issn.1671-167X.2015.01.008
- VernacularTitle:两种钙黏蛋白在小鼠牙齿发育期的时间和空间表达
- Author:
Shuangyun ZHAO
;
Ming LIU
;
Yuming ZHAO
;
Qingjie LIN
;
Xiuping WANG
;
Lihong GE
- Publication Type:Journal Article
- Keywords:
Cadherins;
Tooth germ;
Fluorescent antibody technique
- From:
Journal of Peking University(Health Sciences)
2015;(1):42-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression patterns of E-cadherin and P-cadherin in murine-tooth germs at early developmental stages .Methods:Mandible samples of CD 1 mice from embryonic day 12 .5 to postnatal day 3 .5 were collected .The expressions of E-cadherin and P-cadherin in murine man-dibular first molar germs were detected by immunofluorescence and observed under confocal fluorescence microscope .HE staining was performed for tissue morphology .Results:Both E-cadherin and P-cadherin were widely expressed in the epithelial tissues through early developmental stages .The E-cadherin ex-pression was increased in polarizing pre-ameloblasts , whereas the P-cadherin expression declined .The expression of the P-cadherin could be detected in epithelial tissues before bud stage , and expressed in mature ameloblasts at secretory stage .Conclusion:The E-cadherin and P-cadherin expressed in different spatiotemporal expression patterns , indicating their individual functions during tooth development .P-cad-herin might function in the secretion and mineralization of enamel .
