The expression of Wnt/beta-catenin signaling molecule in inflammatory bowel diseases treated with bone marrow mesenchymal stem cell transplantation
10.3969/j.issn.2095-4344.2015.01.009
- VernacularTitle:骨髓间充质干细胞移植炎症性肠上皮组织中Wnt/β-catenin信号分子的表达
- Author:
Yanfen XING
;
Xuhong XIE
;
Zhaohu YUAN
;
Yejia CUI
;
Yuyuan LI
;
Yuqiang NIE
;
Yaming WEI
- Publication Type:Journal Article
- Keywords:
Bone Marrow;
Mesenchymal Stem Cell Transplantation;
Intestinal Diseases;
Signal Transduction
- From:
Chinese Journal of Tissue Engineering Research
2015;(1):49-53
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The Wnt/β-catenin signaling pathway is one of the most important signaling pathways in stem cel regulation, which is involved in regulation of cel proliferation and differentiation. OBJECTIVE:To investigate the expression of Wnt/β-catenin main signaling molecule in inflammatory bowel tissues treated with bone marrow mesenchymal stem cel transplantation. METHODS:2,4,6-Trinitrobenzene sulfonic acid was used for establishing inflammatory bowel diseases rat models. Bone marrow mesenchymal stem cels labeled with green fluorescent protein were transplanted into rat modelsviatail vein. Normal saline was injected as control. The expression of Wnt/β-catenin signaling molecule was detected in the large intestine tissue of inflammatory bowel disease rat models by quantitative RT-PCR at 14 and 28 days after transplantation. RESULTS AND CONCLUSION:Real-time quantitative PCR results showed that the expression of Wnt3a andβ-catenin in the inflammatory bowel tissue increased significantly (P < 0.05), while no difference in the expression of c-myc (P > 0.05). The expressions of Wnt3a, β-catenin and c-myc in the transplantation group were significantly lower than those in the control group after transplantation (P <0.05). These findings indicate that the Wnt/β-catenin signaling pathway plays important roles in inflammatory bowel disease and repair after bone marrow mesenchymal stem cel transplantation, while this pathway may promote stem cels differentiating into intestinal epithelium, promote recovery from inflammatory bowel disease, repair inflammatory area, and restore intestinal tissue homeostasis.
