Identification of a novel human leucocyte antigen allele B*07:110
10.3969/j.issn.2095-4344.2015.01.024
- VernacularTitle:人类白细胞抗原新等位基因B*07:110的序列分析及确认
- Author:
Yue ZHOU
;
Jianping LI
- Publication Type:Journal Article
- Keywords:
Tissue Engineering;
HLA Antigens;
Aleles;
Nucleic Acid Probes
- From:
Chinese Journal of Tissue Engineering Research
2015;(1):135-139
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:In recent years, with the development of China Marrow Donor Program and the improvement of human leukocyte antigen (HLA) typing technique, novel aleles of human leukocyte antigen have been discovered constantly in China. OBJECTIVE:To identify and confirm a novel HLA alele in a Chinese individual. METHODS: A new HLA alele was found during routine human leukocyte antigen genotyping by PCR-sequence specific oligonucleotide probes and sequencing-based typing. RESULTS AND CONCLUSION:The HLA-B locus hybridization probe reaction patterns of this sample did not match with any known HLA-B aleles or alelic combinations. Exons 2, 3 were sequenced in both directions using HLA-B sequence primer and group-specific sequencing primer. The obtained sequence had 2nts change from B*07:02:01 at nt 226 and nt 228 where A->G (codon 76 ATA->GTG) and resulting in a coding change, 76 isoleucine (I) was changed to valine (V). This nucleotide sequence has been submitted to the GenBank nucleotide sequence database and it is available under the accession number HM989017. A novel HLA alele, HLA-B*07:110, was identified, and was named officialy by the WHO Nomenclature Committee.
