Analysis of sites of Toxoplasma gondii SAG2 gene in blood of HIV-positive people in Lincang City,Yunnan Province

VernacularTitle:云南临沧HIV阳性者血液中弓形虫SAG2基因位点分析
Author: Yuxi JIA ; Lingjuan CHEN ; Wei LI ; Daping NIE ; Mi LUO ; Jianfang HE ; Lijie SHEN
Publication Type:Journal Article
Keywords: Toxoplasma gondii; HIV-positive; SAG2 gene; Genetype; Nested PCR; Sequence analysis
From: Chinese Journal of Schistosomiasis Control 2015;(1):32-35
CountryChina
Language:Chinese
Abstract: Objective To preliminarily understand the genotype characteristics of Toxoplasma gondii in blood of HIV?posi?tive persons in Lincang City,Yunnan Province. Method Two segments of SAG2 gene of T. gondii from blood samples of HIV?positive persons in Lincang City were extracted and amplified by using the nested PCR method and the genotype was identified and compared with the standard strain(Type I)of Toxoplasma gondii. Results Thirty?five SAG2 genes(241 bp)and 35 SAG2 genes(221 bp)of T. gondii were amplified from 170 blood samples of the HIV?positive people,and 4 of each case were selected and digested with enzyme,then 2 aim gene fragments of each case were chosen and compared with the standard strain (Type I)of T. gondii. The digestion of SAG2 gene(241 bp)showed the genotype of the blood samples was Type I or Type II, and the digestion of SAG2 gene(221 bp)confirmed that the genotype was Type I. Conclusion It is preliminarily confirmed that the genotype of T. gondii in blood of HIV?positive persons in Lincang City,Yunnan Province is Type I.