A study of the expression of ANGPT1,ANGPT2 and VEGF in mouse model of acute myeloid Leukemia
10.3969/j.issn.1671-8348.2015.05.004
- VernacularTitle:ANGPT1、ANGPT2、VEGF在急性髓系白血病小鼠模型中的表达研究
- Author:
Hong YANG
;
Fengying TIAN
;
Zeping ZHOU
;
Liang SHAO
;
You ZHANG
- Publication Type:Journal Article
- Keywords:
acute myeloid leukemia;
mouse model;
ANGPT;
vascular endothelial grow th factor
- From:
Chongqing Medicine
2015;(5):586-589
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct mouse model of acute myeloid leukemia and detect the expression of ANGPT 1 ,ANGPT2 and VEGF gene on the cells its as well as the clinical significance .Methods The HL‐60 cells were transfected to NOD/SCID mouse through abdominal injection to construct mouse model of acute myeloid leukemia .Then identify mouse model by histopathology and Flow Cytometry .The expression of ANGPT2 ,ANGPT1 and VEGF mRNA in the tumor tissues of mouse model was detected by real‐time fluorescence quantitative PCR .The expression of ANGPT2 will be analyzed on the survival time of mouse model by Spearman′s correlation method .Results Mouse model has been successfully identified by histopathology and Flow Cytometry .The expression of ANGPT2 and VEGF in mouse mode was significantly detected ,which was that of higher than normal group (P<0 .05) .The expression of ANGPT1 was lower than that of ANGPT2 and VEGF ,there was no significant difference between AN‐GPT1 and normal groups (P>0 .05) .The higher expression of ANGPT2 in mouse model had a short survival time in mouse with acute myeloid leukemia .Conclusion This study showed that ANGPT2 mRNA was over‐expressed in acute myeloid leukemia .The increasing expression of ANGPT2 mRNA may lead to poor prognosis in mouse with acute myeloid leukemia .
