Correlation between interleukin-18 and deep venous thrombosis disease
10.3969/j.issn.1671-8348.2015.05.008
- VernacularTitle:白细胞介素-18与深静脉血栓疾病的相关性研究
- Author:
Guangdi LI
;
En SONG
;
Xueling ZHAO
;
Yuncheng BAI
;
Zhi PENG
;
Rudan ZHOU
- Publication Type:Journal Article
- Keywords:
deep venous thrombosis;
interleukin-18;
biomarker;
human umbilical vein endothelial cells
- From:
Chongqing Medicine
2015;(5):600-604
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between IL‐18 and deep venous thrombosis disease and its clinical significa‐tion .Methods To detect the expression of IL‐18 by ELISA ,we collected the blood samples of DVT patients as the experimental group(n=40) compared to the control group(n=40) and normal group(n=20) .IL‐18 over expression/interference vectors were constructed and transfected human vein endothelial cells ,analyzed by microarray and KEGG Pathway as biology information tech‐nology .Then discuss the association between IL‐18 and DVT .Results Results of ELISA showed that compared with control group and normal group ,the expression of IL‐18 gene in DVT patient were up‐regulated(F=11 .248 ,P<0 .01) .Compared with normal group ,the IL‐18 expression in control group have not been significantly up‐regulated(P>0 .05) .Immunofluorescence detected IL‐18 gene expression in cytoplasm of human umbilical vein endothelial cells (HUVECs) .According to the microarray analysis we found in the IL18‐pCDH‐GFP transfected cells 17 signaling pathways were down‐expressed while 16 signaling pathways were up‐expressed .Compared with normal group cells ,in the IL18‐LMP‐shRNAmir1 transfected cells 23 signaling pathways were down‐ex‐pressed and 9 signaling pathways were up‐expressed .Conclusion Based on the above experimental data ,it is very clear that IL‐18 influenced HUVECs and plays an important role in DVT ,it is possible to predict the diagnosis of DVT and act as candidate molecu‐lar markers .
