Effects of minocycline on neurite outgrowth of PC12 cells following oxygen glucose deprivation
10.3969/j.issn.1671-8348.2015.05.009
- VernacularTitle:米诺环素对PC12细胞缺氧缺糖损伤后神经突生长的影响
- Author:
Tao TAO
;
Xinyue QIN
;
Jinzhou FENG
;
Hua LUO
;
Xiaogang LI
- Publication Type:Journal Article
- Keywords:
neurite outgrowth;
minocycline;
oxygen glucose deprivation;
PC12 cell
- From:
Chongqing Medicine
2015;(5):605-607
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of minocycline on cell viability and neurite outgrowth of pheochromocytoma cells (PC12) after oxygen‐glucose deprivation(OGD) injury .Methods PC12 cells were exposed to OGD insult for 2 ,4 ,6 ,8 h to estab‐lish a cerebral ischemia model in vitro .High‐differentiated PC12 cells were cultivated and randomly divided into three groups :con‐trol group ,OGD group and various doses of minocycline(0 .1 ,1 .0 ,10 .0 μM) treated group .24 h after OGD‐reperfusion ,PC12 cells viability was assessed by CCK‐8 assay ,the neurite was labeled with MAP‐2 by immunofluorescence and neurite length was meas‐ured by the Image‐Pro Plus 7 .0 software ,GAP‐43 protein expression was determined by Western blotting .Results Compared to the OGD groups ,minocycline induced a concentration‐dependent increase in cells viability [(46 .1 ± 2 .9)% vs .(77 .0 ± 2 .5)% ,P<0.01],improvedneuriteoutgrowthandincreasedtheexpressionofGAP‐43proteininPC12cellsafterOGDinjury([(0.34±0.04) vs .(2 .11 ± 0 .10) ,P<0 .01] .Conclusion Minocycline could protect against oxygen glucose deprivation injury and promote neurite outgrowth .This finding suggests minocycline may be a novel therapy for cerebral ischemia .
