Age-associated proliferation and differentiation changes of rat bone marrow mesenchymal stem cells
10.3969/j.issn.2095-4344.2015.01.004
- VernacularTitle:大鼠骨髓间充质干细胞增殖及定向分化能力的增龄性变化
- Author:
Hairui LI
;
Dong ZHENG
;
Can JIANG
;
Jun GUO
;
Aidong ZHANG
;
Zicheng LI
- Publication Type:Journal Article
- Keywords:
Bone Marrow;
Mesenchymal Stem Cells;
Troponin T;
Cell Proliferation
- From:
Chinese Journal of Tissue Engineering Research
2015;(1):18-23
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Autologous bone marrow mesenchymal stem cel transplantation for the treatment of cardiovascular disease has become one of the hotspots, but it is unclear whether the proliferation and directional differentiation of autologous bone marrow mesenchymal stem cels varies changes with age. OBJECTIVE: To explore the proliferation and differentiation changes of rat bone mesenchymal stem cels in different ages. METHODS:The bone marrow mesenchymal stem cels from Sprague-Dawley rats in different age groups were purified and cultured, and then examined by flow cytometry in terms of cel cycle. Meanwhile, neonatal rat cardiomyocytes were co-cultured with bone marrow mesenchymal stem cels. The morphologic changes of bone marrow mesenchymal stem cels and the protein expression of troponin T were detected with immunofluorescence technique. RESULTS AND CONCLUSION: The percentage of bone marrow mesenchymal stem cels in G0/G1 phase was increased with age; while the percentage of expression of troponin T proteins-positive bone marrow mesenchymal stem cels were decreased with age. These findings indicate that the proliferation and differentiation abilities of rat bone marrow mesenchymal stem cels descend with age.
