Preparation and measurement of sodium tanshinone Ⅱ-A sulfonate in mice serum and effects on big conductance calcium-activated potassium channels in porcine coronary artery smooth muscle cells
10.3969/j.issn.1671-8348.2015.04.002
- VernacularTitle:含丹参酮ⅡA 磺酸钠小鼠血清的制备及其对PCASM Cs的BKCa作用的初探
- Author:
Jun CHENG
;
Xiaorong ZENG
;
Pengyun LI
;
Miaoling LI
;
Zhifei LIU
;
Jie PEI
;
Yan YANG
- Publication Type:Journal Article
- Keywords:
salvia miltiorrhita;
sodium tanshinoneⅡ-A sulfonate;
high efficiency liquid chromatography;
serous pharmacology;
patch clamp technique
- From:
Chongqing Medicine
2015;(4):436-438
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss a method combining serum‐pharmacology and electrophysiology technology ,and to research the mechanism of dilating porcine coronary artery of sodium tanshinone Ⅱ‐A sulfonate (DS‐201) .Methods To give mice intragastric administration solution and measure DS‐201 concentration in mice serum ,and apply the serum to single channel patch to research its effect on big conductance calcium‐activated potassium channels(BKca ) in porcine coronary artery smooth muscle cells (PCASMCs) . Results The linear range of concentration containing DS‐201 serum was 0 .73 to 1 .91 μg/mL (r=0 .997 7) ,the recycle rate was 99 .85% -101 .47% ,and the concentration was(7 .32 ± 4 .25)μg/mL ;the result indicates that serum containing DS‐201 has activa‐tion effects on BKCa in PCASMCs ,while there was no statistical significance (P>0 .05) .Conclusion The establishment method of the alcohol extraction of Danshen is useful and reliable .The HPLC method of measuring DS‐201 concentration is precise .Choosing higher quality drugs or raising bioavailability can improve combination of the serum pharmacology and electrophysiological tech‐nique .
