The effects of p38MAPK inhibitor SB203580 on MC3T3-E1 cell proliferation and differetiation under high glucose concentration
10.3969/j.issn.1001-3733.2015.02.08
- VernacularTitle:P38MAPK信号通路抑制剂SB203580对高糖状态下成骨细胞MC3T3-E1增殖和分化的影响
- Author:
Xiaohua YU
;
Shutai LIU
;
Kai DONG
;
Zhonghao LIU
- Publication Type:Journal Article
- Keywords:
Hyperglycemia;
Osteoblast;
Osteogenic differentiation;
P38MAPK
- From:
Journal of Practical Stomatology
2015;(2):184-187
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of p38MAPK inhibitor SB203580 on osteoblast MC3T3-E1 cells under high glu-cose concentration.Methods:MC3T3-E1 cells were exposed to glucose at 5.5 mmol/L,25.5 mmol/L and 25.5 mmol/L combined with SB203580 respectively.Cell proliferation was analysed by MTT assay,calcified nodules were quantitated by Alizarin Red S stai-ning.Osteogenesis gene(OCN,Runx2)mRNA level was examined by realtime PCR.Results:High glucose decreased cell prolifera-tion(P<0.05),ALP activity(P<0.05),calcified nodule formation and osteogenesis gene expression of MC3T3-E1 cells.SB203580 reversed the down-regulation of osteogenic markers under high glucose,increased the ALP expression but decreased cell proliferation. Conclusion:SB203580 is antagonistic to P38MAPK in the regulation of preliferation and differetiation of MC3T3-E1 cells.
