Effect of osteoprotegerin on osteoclasts
- VernacularTitle:骨保护素对破骨细胞的影响程度分析
- Author:
Jian LIU
;
Hui SONG
- Publication Type:Journal Article
- Keywords:
osteoprotegerin;
osteoclasts;
resorption pits
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(3):65-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyse the effects of OPG (osteoprotegerin, OPG) at different concentrations on activity of osteoclasts (osteoclasts, OC) , and the relationship between them in physiological activity and function.Methods The bone marrow cells of six weeks old female mice were collected and cultured in vitro on the basis of adding different levels of OPG(10, 20, 50, 100 μg/L).The relationship between OPG and OC were analysed by tartrate resistant acid phosphatase ( TRAP) staining, F-actin cytoskeleton staining and bone resorption pits.Results OC showed adherent growth within 1h cultured, and there were no large multicore OC.Cultured for 5 days, the smaller monocytes gradually began to fusion with each other to form multinucleated cells and showed distinctive characteristics.After three days OPG treatment, the number of multicore cells gradually reduced with OPG concentration increaseing in experimental group, and showing a negative correlation between them(r=-0.516,P<0.05).And there were only a small amount of multinucleated cells at OPG concentration of 50 μg/L.At 20,50 μg/L of OPG concentration, OC number significantly decreased compared with control group (P<0.05), and when the concentration of OPG was 100μg/L, OC number showed significant reduction (P<0 01).the experimental group OPG bone resorption lacunae area, density and decrease the degree were positively correlated with concentration of OPG (r=0.459;r=0.426;r=0.389,P<0.05), and the area had a significant difference when two groups compared.Conclusion The inhibitive effects of OPG on OC function and activation increase with concentration increasing.
