The effect of type 2 diabetes on collagen content in different organs in OLETF rats
10.3969/j.issn.1006-6187.2015.04.021
- VernacularTitle:2型糖尿病对OLETF大鼠不同器官胶原蛋白含量影响的研究
- Author:
Huimin WANG
;
Yu LIANG
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus,type 2;
Skin;
Femur;
Liver;
Collagen
- From:
Chinese Journal of Diabetes
2015;(4):365-369
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the effect of type 2 diabetes on collagen content in different organsinOLETFrats.Methods 24maleOLETFratsweregivenhigh‐caloriedietfor30weekstoset up the T2DM. At the 30th week ,16 male T2DM OLETF rats were selected as case group ,and 12 LETO rats were used as control group. The skin ,liver ,and femur tissue were collected at the same time. HE and Masson staining and immunohistochemical test were performed to measure the content of collagen and HPLC was used to detect hydroxyproline. Results Compared with the NC group ,DM group showed the collagen fibers were decreased in femoral and skin tissue ,but increased in liver tissue. The collagen content of the skin tissue was significantly lower in DM group than in NC group [(149.9 ± 18.4) vs (266.0 ± 54.8)mg/g ,P<0.01] ,and the collagen content of the femur tissue was also lower in DM group than in NC group [(185.60 ± 19.38) vs (243.27 ± 22.19)mg/g ,P<0.05]. In contrary ,the collagen content of liver tissue was higher in DM group than in NC group [(134.54 ± 18.78) vs (64.52 ± 21.51) mg/g ,P<0.01]. Conclusion The effect of type 2 diabetes on the collagen in different organs is different. The collagen content decreases in the femoral and skin tissue but increases in the liver.
