Establishment and optimization ofα2-macroglobulin activity detection assay
10.7644/j.issn.1674-9960.2015.03.008
- VernacularTitle:α2－巨球蛋白活性检测方法的建立与优化
- Author:
Chaoji HUANGFU
;
Yuyuan MA
;
Yanlin WANG
;
Xiong ZHAO
;
Jingang ZHANG
- Publication Type:Journal Article
- Keywords:
α2-macroglobulin;
Cohn fraction Ⅳ;
trypsin;
trypsin inhibitors;
BAPNA
- From:
Military Medical Sciences
2015;(3):193-195
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an assay for detecting α2-macroglobulin activity in Cohn fraction Ⅳ.Methodsα2-M reacted with trypsin to form α2-M-trypsin complex.After the chromogenic substrate Na-benzoyl-DL-arginine 4-nitroanilide hydrochloride ( BAPNA) was added, absorption at 410 nm was detected with the microplate reader .α2-M activity in Cohn fractionⅣwas quantitatively detected according to the established standard curve of plasma α2-M activity. Result Several critical parameters in this assay were optimized .A standard curve of plasma α2-M activity was established . According to this standard curve ,α2-M activity in Cohn fraction Ⅳsample was detected to be 1.578 PU/ml.Conclusion Using normal human plasma as the reference material , theα2-M activity in Cohn fractionⅣcan be detected through chro-mogenic substrate assay.This study provides a simple method to detect α2-M activity during the purification process of α2-M from Cohn fraction Ⅳ.
