Experimental study of calvarial critical size defect in rats with type 2 diabetes mellitus
10.3969/j.issn.1001-3733.2015.02.02
- VernacularTitle:2型糖尿病大鼠颅骨极限骨缺损的实验研究
- Author:
Lifeng WANG
;
Kaixiu FANG
;
Xiaoru XU
;
Shuai REN
;
Naiwen TAN
;
Zhen LI
;
Lincong QIU
;
Wei MA
;
Dehua LI
;
Yingliang SONG
- Publication Type:Journal Article
- Keywords:
Bone tissue engineering;
Calvarial critical size defect;
Type 2 diabetes mellitus
- From:
Journal of Practical Stomatology
2015;(2):157-161
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the calvarial critical size defect (CSD)in rats with type 2 diabetes mellitus(T2DM).Methods:T2DM model of SD rats(weighted 300-320 g)was induced by high fat and high sugar diet and low dose intraperitoneal streptozotocin (STZ)injection.The rats with T2DMand the normal controls were divided into 4 groups(n=3)respectively.Defects with the diame-ter(mm)of 2,3,4 and 5 were made on the central calvaria of each rat.General observation,X-ray examination and histological study were performed 8 weeks postoperatively.Results:In the T2DM group,only the defects of 2 mm diameter were healed completely,X-ray resistance and new bone formation were observed;the defects of 3,4 and 5 mm diameter were unhealed,X-ray transmission was observed and newly formed bone was insufficient.In the control group,the defects of 2,3 and 4 mm diameter were healed completely, X-ray resistance and new bone formation were observed;the defects of 5 mm diameter were unhealed,X-ray transmission was ob-served,newly formed bone was insufficient.Conclusion:The calvarial CSD of T2DM rat model can be defined as the defect with the diameter of 3 mm.
