Research on Urine Metabolomics of HIV/AIDS Patients with Spleen-lung Qi-deifciency Based on H-NMR Technique
10.11842/wst.2015.02.011
- VernacularTitle:基于氢核磁共振技术HIV/AIDS肺脾气虚证患者尿液代谢组学研究
- Author:
Qianlei XU
;
Guanxiao WEN
;
Juan WANG
;
Shiping XIE
;
Suna MA
;
Ning ZHANG
;
Wei LIU
;
Yongxia CUI
- Publication Type:Journal Article
- Keywords:
H-NMR;
HIV/AIDS;
spleen-lung qi-deficiency pattern;
urine;
metabolomics
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(2):356-361
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the characteristics of urine metabolomics among HIV/AIDS patients with spleen-lung qi-deficiency. H-NMR technique was combined with principal component analysis and cluster analysis in the comparison of urine metabolic products among 24 HIV/AIDS cases with spleen-lung qi-deficiency and 20 healthy control cases. The results showed that urine metabolic products of HIV/AIDS patients with spleen-lung qi-deficiency and healthy people by H-NMR technique detection and PLS-DA analysis can classify the outline of urine metabolites. There were about 20 variables in the difference between two groups. The speculated substances contained glycyl-L-leucine, L-valine, α-aminobutyric acid, methyl succinic acid, glycine propionyl, and etc. It was concluded that H-NMR technique was able to classify the outline of urine metabolites between HIV/AIDS cases with spleen-lung qi-deficiency and healthy people. Part of the potential existed characteristic markers contributed to the clinical diagnosis of traditional Chinese medicine (TCM) syndrome differentiation of AIDS. It had certain effect in its quantitative analysis.
