Methylation status of IGF -Ⅱgene P4 promoter in hepatocellular carcinoma
10.3760/cma.j.issn.1008-6706.2015.06.001
- VernacularTitle:肝癌组织中胰岛素样生长因子Ⅱ基因P4启动子甲基化状态及其作用研究
- Author:
Li PING
;
Yaoxing HUANG
;
Yanlei DU
;
Qiong WU
;
Jixiang PENG
- Publication Type:Journal Article
- Keywords:
Liver Neoplasms;
Insulin-Like Growth Factor Ⅱ;
Transcription Initiation Site;
Methylation
- From:
Chinese Journal of Primary Medicine and Pharmacy
2015;(6):801-803
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the alternation of insulin -like growth factor -Ⅱ( IGF-Ⅱ) gene promoter P4 methylation status in hepatocellular carcinoma ( HCC) and explore its relationship with expression of P4 mRNA levels.Methods Liver specimens of 43 patients with HCC and normal liver specimens of 9 control patients were collected in operation .Tissue DNA and total RNA were extracted from these specimens .IGF-Ⅱ P4 methylation status and P4 mRNA expression levels were detected .Results (1)The incidence of IGF -ⅡP4 methyl-ation in HCC group was significantly lower than that in normal liver specimens (16.28%vs 88.89%,χ2 =19.12,P<0.01).(2)The expression level of IGF -ⅡP4 mRNA in HCC group was significantly higher than that that in normal liver specimens[(0.96 ±0.74) vs (0.25 ±0.19),t=5.48,P<0.01].(3)In HCC group,the IGF-Ⅱ P4 mRNA expression level with hypomethylation gene was significantly higher than that without hypomethylation gene [(1.18 ± 0.76) vs (0.32 ±0.27),t=5.28,P<0.01].Conclusion The hypomethylation alternation of IGF -Ⅱ P4 gene promoter which is accomplished by up -regulate P4 mRNA expression has a close relationship with HCC .
