Establishment and application of viral inactivation/removal technology for blood products
10.7644/j.issn.1674-9960.2015.03.002
- VernacularTitle:血液制品病毒灭活／去除技术平台的建立与应用
- Author:
Huiqiong YIN
;
Rui WANG
;
Fengxuan ZHU
;
Jianguo WANG
;
Jingang ZHANG
- Publication Type:Journal Article
- Keywords:
blood products;
viral inactivation;
disinfection
- From:
Military Medical Sciences
2015;(3):165-168
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish viral inactivation/removal techniques for blood products , and apply them to inacti-vation/removal process validation of blood products .Methods Enveloped and non-enveloped model viruses were propaga-ted.Viral inactivation/removal techniques for blood products ,including solvent/detergent (S/D) treatment, low pH incuba-tion, dry heat method, pasteurization,and nanofiltration, were established.The virus titer was evaluated using cytopathic effects ( CPE) and Spearman and Karber method .The viral inactivation/removal techniques were believed to be effective when LRV≥4.These techniques were used in viral inactivation /removal validation of blood products .Results Enveloped model viruses were inactivated through S/D treatment and the low pH incubation method .Enveloped and non-enveloped model viruses were inactivated through dry heat and pasteurization .Within a certain range of filtration capacity , PPV was removed through nanofiltration .Conclusion The established viral inactivation/removal techniques can be used in viral inactivation/removal process validation of blood products , which can improve viral safety of blood products .
