Contamination of human parvovirus B19 in source plasma and coagulation factor products

VernacularTitle:原料血浆和凝血因子类产品中人细小病毒B19污染情况调查
Author: Junting JIA ; Yuyuan MA ; Yi GUO ; Xiong ZHAO ; Fuguang ZHAO ; Jingang ZHANG
Publication Type:Journal Article
Keywords: human parvovirus B19; plasma; coagulation factor products; TaqMan real-time quantitative PCR
From: Military Medical Sciences 2015;(3):169-173
CountryChina
Language:Chinese
Abstract: Objective To detect human parvovirus B19(B19V)DNA in source plasma pools and coagulation factor products and determine its prevalence and the level of contamination .Methods A pair of primers and a probe selected from the highly conserved sequences encoding the non-structural protein(NS1)of B19 were designed and synthesized.With the primer-probe combination ,source plasma pools and four types of coagulation factor products were determined for B 19V DNA by TaqMan real-time quantitative PCR.Results One-hundred and sixteen from 195 (59.49%) source plasma pools contained B19 DNA and concentrations up to 1.35 ×1010 copies/ml were measured.High frequencies of contamination were detected in factor Ⅷ (29 of 31; 93.55%), thrombin (10 of 10; 100%), fibrinogen (6 of 7; 85.71%) and prothrombin complex (8 of 9;88.89%).Conclusion These data show that B19V is a common contaminator in Chinese source plasma pools and coagulation factor products .Thus,B19V screening in Chinese source plasma seems desirable and significant for the safety of plasma derivatives in China .