Construction of pEGFP-C2-AQP4-M23 plasmid and the effect of AQP4 on astrocytes in hypoglycemia
10.3969/j.issn.1006-5725.2015.05.003
- VernacularTitle:水通道蛋白4重组质粒的构建及其在体外对低糖反应的观察
- Author:
Xiaoyan YU
;
Jiangshan DENG
;
Yuwu ZHAO
- Publication Type:Journal Article
- Keywords:
Aquaporin 4;
EGFP;
Transfection;
Edema
- From:
The Journal of Practical Medicine
2015;(5):700-704
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct pEGFP-C2-AQP4-M23 plasmid and to detect its expression in CTX-TNA2 cells in order to study the effect of AQP4 on astrocytes in hypoglycemia. Methods AQP4 gene obtained from SD rats by RT-PCR was cloned into pEGFP-C2 plasmid. The recombinant plasmid was transfected into CTX-TNA2 cells, then treated with low glucose. The expression and effect of AQP4 on CTX-TNA2 cells were examed with confocol. Results PCR and enzyme digestion showed AQP4 size was right; confocol demonstrated that AQP4 was expressed on the membrane of CTX-TNA2 cells, and the surfacial area of CTX-TNA2 cells with AQP4was larger than those without AQP4 in hypoglycemia. Conclusion pEGFP-C2-AQP4-M23 plasmid was successfully constructed and expressed in the CTX-TNA2 cell membrane and AQP4 plays an important role in cell edema in hypoglycemia.
